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Response: HTTP/1.1 200 OK Content-Type: application/vnd.mendeley-document.1+json Date: Mon, 18 May 2026 17:39:58 GMT Vary: Accept-Encoding Vary: Accept-Encoding X-Mendeley-Trace-Id: 1e3MIDCJ4SI Content-Length: 93 Connection: keep-alive [{"id":"6c3b9b0d-a976-390a-b9e7-54bac332ed6b"},{"id":"49e2533e-f56e-3b38-b03f-04d84cd1d78d"}]

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Response: HTTP/1.1 200 OK Content-Type: application/vnd.mendeley-document.1+json Date: Mon, 18 May 2026 17:39:58 GMT Vary: Accept-Encoding Vary: Accept-Encoding X-Mendeley-Trace-Id: J-KCp_gK5iM Content-Length: 2382 Connection: keep-alive {"title":"Development of specific PCR primers for identification and detection of Rhizopycnis vagum","type":"journal","authors":[{"first_name":"S.","last_name":"Ghignone"},{"first_name":"G.","last_name":"Tamietti"},{"first_name":"M.","last_name":"Girlanda"}],"year":2003,"source":"European Journal of Plant Pathology","identifiers":{"issn":"09291873","scopus":"2-s2.0-0242653865","doi":"10.1023/A:1026171307719"},"keywords":["Dark sterile mycelia","PCR diagnostics","Ribosomal DNA","Vine collapse"],"volume":"109","issue":"8","id":"6c3b9b0d-a976-390a-b9e7-54bac332ed6b","created":"2018-11-22T13:27:20.768Z","file_attached":false,"profile_id":"25727314-8dbc-3d3c-847b-a3bb8a0f1c92","last_modified":"2023-02-08T16:06:20.118Z","read":false,"starred":true,"authored":true,"confirmed":false,"hidden":false,"folder_uuids":["5b7b60dd-713c-4196-a51f-a75fed10561c"],"private_publication":false,"abstract":"Rhizopycnis vagum is a recently described coelomycete known to belong to the complex of root rot pathogens contributing to vine decline of cucurbits in several parts of the world. However, the fungus has also been reported to infect tomato, and as an endophytic associate of mycorrhizal roots of wild, asymptomatic Pinus halepensis and Rosmarinus officinalis plants in Italy. To accelerate epidemiological and ecological investigations on this fungus, a PCR primer pair was developed. Primers Rv1-F and Rv1-R were designed, based on alignment of internal transcribed spacer (ITS) sequences (ITS1-5.8S-ITS2), which amplified a 396-bp fragment from all R. vagum isolates tested, including isolates pathogenic to melons and endophytic isolates from mycorrhizae. Specificity of the primer pair was verified both in silico (BLAST searches using each primer string as a query) and in PCR assays, where the primers failed to amplify DNA from any isolate of fungi taxonomically related to R. vagum (e.g. Massarina walkeri and Stagonospora spp.) and other vine decline and common soilborne pathogens (e.g. Monasporascus cannonballus, Acremonium cucurbitacearum, Fusarium spp. and Rhizoctonia solani). Under optimum conditions, detectable amplification of the specific sequence required 0.05 pg of target DNA. Amplification of the expected 369-bp fragment was also obtained from DNA root extracts of nearly asymptomatic Cucumis melo plants inoculated with R. vagum under greenhouse conditions."}

Request: https://api.mendeley.com/documents/49e2533e-f56e-3b38-b03f-04d84cd1d78d?view=all

Response: HTTP/1.1 200 OK Content-Type: application/vnd.mendeley-document.1+json Date: Mon, 18 May 2026 17:39:59 GMT Vary: Accept-Encoding Vary: Accept-Encoding X-Mendeley-Trace-Id: Hnx8-CCdwfE Content-Length: 2344 Connection: keep-alive {"title":"Development and evaluation of a real-time PCR seed lot screening method for Fusarium circinatum, causal agent of pitch canker disease","type":"journal","authors":[{"first_name":"T J","last_name":"Dreaden"},{"first_name":"J A","last_name":"Smith"},{"first_name":"E L","last_name":"Barnard"},{"first_name":"G","last_name":"Blakeslee"}],"year":2012,"source":"Forest Pathology","identifiers":{"doi":"https://doi.org/10.1111/j.1439-0329.2012.00774.x"},"pages":"405-411","volume":"42","issue":"5","websites":["https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1439-0329.2012.00774.x"],"id":"49e2533e-f56e-3b38-b03f-04d84cd1d78d","created":"2023-02-09T13:56:53.888Z","file_attached":false,"profile_id":"25727314-8dbc-3d3c-847b-a3bb8a0f1c92","last_modified":"2023-02-09T13:56:54.129Z","read":false,"starred":false,"authored":false,"confirmed":true,"hidden":false,"citation_key":"https://doi.org/10.1111/j.1439-0329.2012.00774.x","source_type":"article","folder_uuids":["5b7b60dd-713c-4196-a51f-a75fed10561c"],"private_publication":false,"abstract":"Summary Fusarium circinatum is a serious pathogen of Pinus spp. worldwide, causing pitch canker disease. F. circinatum can contaminate seeds both internally and externally and is readily disseminated via contaminated seed. Many countries require screening of pine seeds for F. circinatum before they can be imported. The currently accepted screening method is based on culturing the pathogen on a semi-selective medium and identifying it using morphological traits. This method is time-consuming and does not allow for accurate identification of the pathogen to the species level. A bulk DNA extraction and real-time PCR procedure to screen seeds for the presence of F. circinatum were developed in this study. The real-time PCR method resulted in the detection of F. circinatum in 5 of 6 commercial seed lots tested and has a lower detection limit of 1 × 10−5 ng of F. circinatum DNA per PCR. The culture-based method detected Fusarium spp. in four of six of the same seed lots. The real-time PCR method can be used to screen multiple seed lots in 2 days, whereas the culture-based method requires a minimum of 1–2 weeks. This new real-time PCR seed screening method allows for fast, sensitive and accurate screening and can be adapted to handle larger volumes of seeds."}

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2012<\/h2>

T J Dreaden, J A Smith, E L Barnard, G Blakeslee<\/span> (2012)<\/span> Development and evaluation of a real-time PCR seed lot screening method for Fusarium circinatum, causal agent of pitch canker disease<\/span>, Forest Pathology<\/span> 42<\/span>(5)<\/span>, p. 405-411<\/span>, url<\/span><\/a><\/span>, doi:https:\/\/doi.org\/10.1111\/j.1439-0329.2012.00774.x<\/span><\/a><\/span>
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2003<\/h2>

S. Ghignone, G. Tamietti, M. Girlanda<\/span> (2003)<\/span> Development of specific PCR primers for identification and detection of Rhizopycnis vagum<\/span>, European Journal of Plant Pathology<\/span> 109<\/span>(8)<\/span>, doi:10.1023\/A:1026171307719<\/span><\/a><\/span>
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2012<\/p>\n

T J Dreaden, J A Smith, E L Barnard, G Blakeslee (2012) Development and evaluation of a real-time PCR seed lot screening method for Fusarium circinatum, causal agent of pitch canker disease, Forest Pathology 42(5), p. 405-411, url, doi:https:\/\/doi.org\/10.1111\/j.1439-0329.2012.00774.x<\/p>\n

2003<\/p>\n

S. Ghignone, G. Tamietti, M. Girlanda (2003) Development of specific PCR primers for identification and detection of Rhizopycnis vagum, European Journal of Plant Pathology 109(8), doi:10.1023\/A:1026171307719<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":7,"comment_status":"open","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/pages\/522"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=522"}],"version-history":[{"count":31,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/pages\/522\/revisions"}],"predecessor-version":[{"id":1683,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/pages\/522\/revisions\/1683"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=522"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}