{"id":1012,"date":"2012-06-26T15:15:26","date_gmt":"2012-06-26T14:15:26","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1012"},"modified":"2012-08-31T15:11:32","modified_gmt":"2012-08-31T14:11:32","slug":"development-and-verification-of-a-diagnostic-assay-based-on-ef-1-%ce%b1-for-the-identification-of-armillaria-species-in-northern-europe","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1012","title":{"rendered":"Development and verification of a diagnostic assay based on EF-1 \u03b1 for the identification of Armillaria species in Northern Europe"},"content":{"rendered":"

See on Scoop.it<\/a> – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI<\/a>
<\/a><\/p>\n

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The overall aim of this study was to develop a new, reliable and rapid diagnostic assay for differentiating six European Armillaria<\/em> species based on variation in their elongation factor-1 alpha (EF-1 \u03b1) gene sequences and to verify a set of species-specific primers on 61 Armillaria isolates from Europe. Partial sequences of the EF-1 \u03b1 gene obtained in Armillaria borealis<\/em>, Armillaria cepistipes<\/em>, Armillaria gallica<\/em>, Armillaria mellea<\/em>, Armillaria ostoyae<\/em> and Armillaria tabescens<\/em> revealed sufficient interspecific variation to distinguish among species using nested primers. These primers gave unambiguous bands when tested on representative isolates of five of these species. However, the EF-1 \u03b1 sequences of European A. borealis isolates clustered into two distinct clades, termed here AbX and AbY. Specific primers were subsequently designed and tested successfully on both AbX-type and AbY-type A. borealis<\/em> isolates. The taxonomy of A. borealis<\/em> needs to be elucidated to determine whether a new, as yet unnamed Armillaria taxon exists in Europe. Three A. borealis isolates were also found to have heterozygous sites in their EF-1 \u03b1 sequences, which suggests that the gene could exist in more than one copy or that these isolates contain hybrid sequences. A pyrosequencing method was also developed, targeting a small region of EF-1 \u03b1 intron 4, which was able to differentiate European Armillaria<\/em> isolates to the species level and additionally could distinguish AbX-type and AbY-type A. borealis<\/em> isolates.<\/p>\n

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V. Mulholland, G. A. MacAskill, B. E. Laue, H. Steele, D. Kenyon, S. Green<\/strong>
\nForest Pathology
Volume 42, Issue 3, pages 229–238, June 2012
\nhttp:\/\/dx.doi.org\/10.1111\/j.1439-0329.2011.00747.x<\/a><\/p>\n

See on onlinelibrary.wiley.com<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI<\/p>\n

The overall aim of this study was to develop a new, reliable and rapid diagnostic assay for differentiating six European Armillaria species based on variation in their elongation factor-1 alpha (EF-1 \u03b1) gene sequences and to verify a set of species-specific primers . . . → Read More: Development and verification of a diagnostic assay based on EF-1 \u03b1 for the identification of Armillaria species in Northern Europe<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[72,15,21,11,10],"tags":[144,23,16,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1012"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1012"}],"version-history":[{"count":11,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1012\/revisions"}],"predecessor-version":[{"id":1040,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1012\/revisions\/1040"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1012"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1012"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1012"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}