{"id":1094,"date":"2013-01-07T10:52:38","date_gmt":"2013-01-07T09:52:38","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1094"},"modified":"2013-01-07T11:11:44","modified_gmt":"2013-01-07T10:11:44","slug":"the-principle-of-lamp-method","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1094","title":{"rendered":"The principle of LAMP method"},"content":{"rendered":"<p><a href='http:\/\/loopamp.eiken.co.jp\/e\/lamp\/index.html' target=\"_blank\">[ Eiken GENOME SITE ] &#8211; The principle of LAMP method<\/a>.<\/p>\n<div class=\"textimage\">\n<p class=\"textimage\"><img decoding=\"async\" src=\"http:\/\/loopamp.eiken.co.jp\/e\/lamp\/img\/primer_1.gif\" alt=\"\" width='500'>&#8220;LAMP&#8221; which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on the target gene and the reaction process proceeds at a constant temperature using strand displacement reaction. Amplification and detection of gene can be completed in a single step, by incubating the mixture of samples, primers, DNA polymerase with strand displacement activity and substrates at a constant temperature (about 65\u00b0C). It provides high amplification efficiency, with DNA being amplified 109-1010 times in 15-60 minutes. Because of its high specificity, the presence of amplified product can indicate the presence of target gene.<\/p>\n<div class=\"clearer\"><\/div>\n<p>Check out the <a href=\"http:\/\/loopamp.eiken.co.jp\/e\/lamp\/principle.html\" target=\"_blank\">basic principle explanation<\/a> and the <a href=\"http:\/\/loopamp.eiken.co.jp\/e\/lamp\/anim.html\" target=\"_blank\">animation<\/a> on the Eiken Genome Site.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[ Eiken GENOME SITE ] &#8211; The principle of LAMP method.<\/p>\n<p class=\"textimage\">&#8220;LAMP&#8221; which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1094\">The principle of LAMP method<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[81,22],"tags":[23,78,79,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1094"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1094"}],"version-history":[{"count":24,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1094\/revisions"}],"predecessor-version":[{"id":1119,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1094\/revisions\/1119"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1094"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1094"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1094"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}