{"id":1136,"date":"2013-01-16T12:46:56","date_gmt":"2013-01-16T11:46:56","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1136"},"modified":"2013-01-16T12:46:56","modified_gmt":"2013-01-16T11:46:56","slug":"development-of-a-pcr-assay-to-detect-the-potential-production-of-nivalenol-in-fusarium-poae","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1136","title":{"rendered":"Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/sppadbase.ipp.cnr.it\/?attachment_id=1322\" rel=\"attachment wp-att-1322\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/sppadbase.ipp.cnr.it\/wordpress\/wp-content\/uploads\/2013\/01\/fml2581-fig-0001-300x91.png\" alt=\"fml2581-fig-0001\" width=\"300\" height=\"91\" class=\"alignnone size-medium wp-image-1322\" srcset=\"https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/fml2581-fig-0001-300x91.png 300w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/fml2581-fig-0001-150x45.png 150w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/fml2581-fig-0001-400x122.png 400w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/fml2581-fig-0001.png 418w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><em>Fusarium<\/em> species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of <em>Fusarium poae<\/em> has increased within the <em>Fusarium<\/em> head blight complex. <em>Fusarium poae<\/em> is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic effects. In this study, a specific primer pair was designed based on the <em>tri<\/em>7 gene to detect potential nivalenol-producing <em>F.\u00a0poae<\/em> isolates. A total of 125 <em>F.\u00a0poae<\/em>, four <em>F.\u00a0cerealis<\/em>, two <em>F.\u00a0culmorum<\/em>, one <em>F.\u00a0langsethiae<\/em>, one <em>F.\u00a0sporotrichioides<\/em> and seven <em>F.\u00a0graminearum<\/em>, plus <em>F.\u00a0austroamericanum<\/em>, <em>F.\u00a0meridionale<\/em>, <em>F.\u00a0graminearum sensu stricto<\/em> and <em>F.\u00a0cortaderiae<\/em> from the NRRL collection were analysed, and only <em>F.\u00a0poae<\/em> isolates gave a positive result for the presence of a 296-bp partial tri7 DNA fragment. Moreover, the primer set was tested from cereal seed samples where <em>F.\u00a0poae<\/em> and other <em>Fusarium<\/em> species with a negative result for the specific reaction (<em>F.\u00a0graminearum<\/em>, <em>F.\u00a0oxysporum<\/em>, <em>F.\u00a0chlamydosporum<\/em>, <em>F.\u00a0sporotrichioides<\/em>, <em>F.\u00a0equiseti<\/em> and <em>F.\u00a0acuminatum<\/em>) were isolated, and the expected fragment was amplified. We developed a rapid and reliable PCR assay to detect potential nivalenol-producing <em>F.\u00a0poae<\/em> isolates.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Mar\u00eda I. Dinolfo, Germ\u00e1n G. Barros, Sebasti\u00e1n A. Stenglein<\/strong><br \/>\nFEMS Microbiology Letters<br \/>\nVolume 332, Issue 2, pages 99\u2013104, July 2012<\/p>\n<p>via <a href='http:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1574-6968.2012.02581.x\/full'>Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae &#8211; Dinolfo &#8211; 2012 &#8211; FEMS Microbiology Letters &#8211; Wiley Online Library<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1136\">Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[14,100,15,42,3,99,11,10],"tags":[23,16,125,160,129,159,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1136"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1136"}],"version-history":[{"count":5,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1136\/revisions"}],"predecessor-version":[{"id":1406,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1136\/revisions\/1406"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1136"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}