{"id":1154,"date":"2013-01-11T15:30:05","date_gmt":"2013-01-11T14:30:05","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1154"},"modified":"2013-01-11T15:31:18","modified_gmt":"2013-01-11T14:31:18","slug":"early-detection-of-gray-mold-in-grape-using-conventional-and-molecular-methods-2","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1154","title":{"rendered":"Early detection of gray mold in grape using conventional and molecular methods"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/www.academicjournals.org\/AJB\/contents\/2012cont\/25Oct.htm\" target=\"_blank\"><img decoding=\"async\" src=\"http:\/\/www.academicjournals.org\/AJB\/cover\/2012\/25Oct.jpg\" alt=\"Table of Contents: 25 October 2012; 11(86)\" \/><\/a><em>Botrytis cinerea<\/em> affects grape quality and yield, and can be difficult to manage due in part to non-symptomatic, quiescent infection in berry development. The aim of this study was to develop a dual system for the detection, isolation and quantification of <em>B. cinerea<\/em>. After three days of samples replication on the modified selective medium (mKERS), the results showed a significant infection effect on the majority of inflorescence samples, especially on the small berries which demonstrated Botrytis infection in all tested plants and appeared to be highly susceptible to <em>Botrytis<\/em> infection prior to harvest. Moreover, infection variation was determined in almost all inflorescence samples taken from different plants. The real-time PCR assay was used to determine the DNA quantity of B. cinerea in each sample tested. A linear relationship was found in these two systems, conventional and molecular assays, to demonstrate the infection of different samples with <em>B. cinerea<\/em>. Although, the real-time PCR assay was highly expensive, it appeared to be more rapid and sensitive than the conventional selective medium assay, allowing both detection and quantification of B. cinerea within 3 h. However, conventional assay has an advantage of both detection and isolation of viable cells of <em>B. cinerea<\/em>, which resulted in making a wide collection of different isolates. Furthermore, this conventional assay is cheaper than molecular test, especially when we carry out a routine work. This dual method proved to be selective and sensitive assays and should be used to monitor\u00a0<em>Botrytis<\/em> infection in the field.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Hala Abdel Wahab and Rania A. A. Younis<\/strong><br \/>\nAfrican Journal of Biotechnology Vol. 11(86), pp. 15251-15257<br \/>\n25 October 2012<br \/>\nDOI: <a href='http:\/\/dx.doi.org\/10.5897\/AJB12.1079' target='blank_'>10.5897\/AJB12.1079<\/a><\/p>\n<p>via <a href='http:\/\/www.academicjournals.org\/AJB\/abstracts\/abs2012\/25Oct\/Wahab%20and%20Younis.htm' target=\"_blank\">Wahab and Younis. Early detection of gray mold in grape using conventional and molecular methods. Afr. J. Biotechnol.<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Botrytis cinerea affects grape quality and yield, and can be difficult to manage due in part to non-symptomatic, quiescent infection in berry development. The aim of this study was to develop a dual system for the detection, isolation and quantification of B. cinerea. After three days of samples replication on the modified selective <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1154\">Early detection of gray mold in grape using conventional and molecular methods<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[82,83,15,3,11,10],"tags":[150,23,16,116,58,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1154"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1154"}],"version-history":[{"count":20,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1154\/revisions"}],"predecessor-version":[{"id":1174,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1154\/revisions\/1174"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1154"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1154"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1154"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}