{"id":1187,"date":"2013-01-11T17:51:27","date_gmt":"2013-01-11T16:51:27","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1187"},"modified":"2013-01-11T17:59:28","modified_gmt":"2013-01-11T16:59:28","slug":"development-of-a-quantum-dots-fret-based-biosensor-for-efficient-detection-of-polymyxa-betae","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1187","title":{"rendered":"Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/sppadbase.ipp.cnr.it\/?attachment_id=1205\" rel=\"attachment wp-att-1205\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/sppadbase.ipp.cnr.it\/wordpress\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g-300x154.jpeg\" alt=\"tcjp_a_709885_o_f0001g\" width=\"300\" height=\"154\" class=\"alignnone size-medium wp-image-1205\" srcset=\"https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g-300x154.jpeg 300w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g-1024x528.jpeg 1024w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g-150x77.jpeg 150w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g-400x206.jpeg 400w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/tcjp_a_709885_o_f0001g.jpeg 1200w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a>Rhizomania is the most destructive disease in sugar beet throughout the world. The disease is caused by Beet necrotic yellow vein virus (BNYVV). <em>Polymyxa betae<\/em> (Keskin) is the only known vector of BNYVV, for transmission of the virus between the plants. Developing molecular diagnostic methods has a major impact on forecasting and epidemiological studies as well as screening sugar beet plants used in resistance breeding programmes. In the present study, quantum dots (QDs) were biofunctionalized with a specific antibody against P. betae. The glutathione-S-transferase protein&#8217;s (GST) corresponding antibody (anti-GST) was effectively conjugated to Tioglicolicacid-modified Cadmium-Telluride QDs (CdTe-QDs) synthesized in an aqueous solution via electrostatic interaction. The dye (Rhodamine) molecules were attached to the GST. Donor\u2013acceptor complexes (QDs-Ab-GST-Rhodamine) were then formed based on the antigen\u2013antibody interaction. The mutual affinity of the antigen and the antibody brought the CdTeQDs and rhodamine together close enough to allow the resonance dipole\u2013dipole coupling required for fluorescence resonance energy transfer (FRET) to occur. The immunosensor constructed showed a high sensitivity and specificity of 100%, acceptable stability and could be used for real sample detection with consistent results. To the best of our knowledge, this is the first report on designing a nano-based biosensing tool for the detection of plant pathogenic fungi.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Hossein Safarpour, Mohammad Reza Safarnejad, Meisam Tabatabaei, Afshin Mohsenifar, Fatemeh Rad, Marzieh Basirat, Fatemeh Shahryari &#038; Fatemeh Hasanzadeh<br \/>\n<\/strong>Canadian Journal of Plant Pathology<br \/>\nVolume 34, Issue 4, 2012, pages 507-515<\/p>\n<p>DOI:10.1080\/07060661.2012.709885<\/p>\n<p>via <a href='http:\/\/www.tandfonline.com\/doi\/full\/10.1080\/07060661.2012.709885'>Taylor &amp; Francis Online :: Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae &#8211; Canadian Journal of Plant Pathology &#8211; Volume 34, Issue 4<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Rhizomania is the most destructive disease in sugar beet throughout the world. The disease is caused by Beet necrotic yellow vein virus (BNYVV). Polymyxa betae (Keskin) is the only known vector of BNYVV, for transmission of the virus between the plants. Developing molecular diagnostic methods has a major impact on forecasting and epidemiological <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1187\">Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[15,3,11,84,85],"tags":[23,16,116,58,19,151,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1187"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1187"}],"version-history":[{"count":18,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1187\/revisions"}],"predecessor-version":[{"id":1203,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1187\/revisions\/1203"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1187"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1187"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1187"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}