{"id":1236,"date":"2013-01-14T17:16:44","date_gmt":"2013-01-14T16:16:44","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1236"},"modified":"2013-01-14T17:18:09","modified_gmt":"2013-01-14T16:18:09","slug":"conventional-and-real-time-pcr-for-the-identification-of-fusarium-fujikuroi-and-fusarium-proliferatum-from-diseased-rice-tissues-and-seeds","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1236","title":{"rendered":"Conventional and real-time PCR for the identification of Fusarium fujikuroi and Fusarium proliferatum from diseased rice tissues and seeds"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/sppadbase.ipp.cnr.it\/?attachment_id=1242\" rel=\"attachment wp-att-1242\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/sppadbase.ipp.cnr.it\/wordpress\/wp-content\/uploads\/2013\/01\/amatulli-222x300.png\" alt=\"amatulli\" width=\"222\" height=\"300\" class=\"alignnone size-medium wp-image-1242\" srcset=\"https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/amatulli-222x300.png 222w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/amatulli-760x1024.png 760w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/amatulli-111x150.png 111w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/amatulli-400x538.png 400w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/amatulli.png 980w\" sizes=\"(max-width: 222px) 100vw, 222px\" \/><\/a><em>Fusarium fujikuroi<\/em> is a species of the <em>Gibberella fujikuroi<\/em> species complex (GFSC) and the causal agent of bakanae disease on rice. Even if F. fujikuroi is the most abundant Fusarium species found on rice, other species can also be isolated from rice, such as <em>F. proliferatum<\/em>. Multiple alignment of translation elongation factor (TEF) gene sequences of different <em>Fusarium<\/em> spp., showed a deletion of six nucleotides in <em>F. fujikuroi<\/em> sequence and a two nucleotide polymorphism in the same region of <em>F. proliferatum<\/em> sequence. These elements of variability were used to develop a conventional and Real-Time PCR assay for diagnosis. The species specific primer pairs (Fuji1F\/TEF1R and Proli1F\/TEF1R) gave a product of 179 and 188\u00a0bp for <em>F. fujikuroi<\/em> and <em>F. proliferatum<\/em> respectively. Primer specificity was confirmed by analyzing the DNA of the most representative species of the GFSC and 298 strains of <em>Fusarium<\/em> spp. isolated from rice plants and seeds in Italy. The specific primers were also successfully used to detect fungal presence directly from infected rice tissues and seeds, providing a rapid tool for the early detection of pathogen contamination.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Maria Teresa Amatulli, Davide Spadaro, Maria Lodovica Gullino and Angelo Garibaldi<\/strong><br \/>\nEuropean Journal of Plant Pathology<br \/>\nOctober 2012, Volume 134, Issue 2, pp 401-408<br \/>\nDOI: 10.1007\/s10658-012-9998-0<\/p>\n<p>via <a href='http:\/\/link.springer.com\/article\/10.1007\/s10658-012-9998-0'>Conventional and real-time PCR for the identification of Fusarium fujikuroi and Fusarium proliferatum from diseased rice tissues and seeds<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Fusarium fujikuroi is a species of the Gibberella fujikuroi species complex (GFSC) and the causal agent of bakanae disease on rice. Even if F. fujikuroi is the most abundant Fusarium species found on rice, other species can also be isolated from rice, such as F. proliferatum. Multiple alignment of translation elongation factor (TEF) <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1236\">Conventional and real-time PCR for the identification of Fusarium fujikuroi and Fusarium proliferatum from diseased rice tissues and seeds<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[14,90,91,15,3,11],"tags":[23,16,154,155,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1236"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1236"}],"version-history":[{"count":12,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1236\/revisions"}],"predecessor-version":[{"id":1249,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1236\/revisions\/1249"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}