{"id":1252,"date":"2013-01-14T17:46:42","date_gmt":"2013-01-14T16:46:42","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1252"},"modified":"2013-01-14T17:47:16","modified_gmt":"2013-01-14T16:47:16","slug":"development-of-specific-markers-for-identification-of-indian-isolates-of-fusarium-oxysporum-f-sp-ricini","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1252","title":{"rendered":"Development of specific markers for identification of Indian isolates of Fusarium oxysporum f.sp. ricini"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/sppadbase.ipp.cnr.it\/?attachment_id=1253\" rel=\"attachment wp-att-1253\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/sppadbase.ipp.cnr.it\/wordpress\/wp-content\/uploads\/2013\/01\/f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ-265x300.jpg\" alt=\"f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ\" width=\"265\" height=\"300\" class=\"alignnone size-medium wp-image-1253\" srcset=\"https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ-265x300.jpg 265w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ-132x150.jpg 132w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ-400x451.jpg 400w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/f6GOTT5pTT2cA-E50BLSYzl72eJkfbmt4t8yenImKBVaiQDB_Rd1H6kmuBWtceBJ.jpg 455w\" sizes=\"(max-width: 265px) 100vw, 265px\" \/><\/a><em>Fusarium oxysporum<\/em> f. sp. <em>ricini<\/em> (<em>F. o. ricini<\/em>) is a ubiquitous soil borne pathogen which causes wilt disease on castor (<em>Ricinus communis<\/em> L). Rapid and reliable detection of the pathogen is essential for undertaking appropriate and timely disease management measures. Identification based on cultural, morphological characteristics and pathogenicity tests are time-consuming and laborious. Traditional methods are now being increasingly replaced by molecular detection techniques, which are much faster and more specific. In this study we have identified two RAPD markers of 1100\u00a0bp and 1350\u00a0bp in size which can be amplified by OPJ-14 and OPK-12 primers respectively for detection of <em>F. o. ricini<\/em>. These two fragments were fully sequenced and two pairs of SCAR primers (For-J14 Fwd\/Rev and For-K12 Fwd\/Rev) were designed. The specific primer pairs amplified a single band from all <em>F. o. ricini<\/em> isolates and there was no amplification from another thirteen <em>Fusarium<\/em> species \/ subspecies tested. These results clearly demonstrate that the designed SCAR primer pairs can be used consistently to detect <em>F. o. ricini<\/em> isolates, isolated from the diseased samples or soil samples. To our knowledge this is the first report on generation of SCAR markers for identification of Indian <em>F. o. ricini<\/em> isolates.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>J. Madhusudhana Reddy, M. A. Raoof, K. Ulaganathan<\/strong><br \/>\nEuropean Journal of Plant Pathology<br \/>\nDecember 2012, Volume 134, Issue 4, pp 713-719<\/p>\n<p>DOI:10.1007\/s10658-012-0047-9<\/p>\n<p>via <a href='http:\/\/link.springer.com\/article\/10.1007\/s10658-012-0047-9'>Development of specific markers for identification of Indian isolates of Fusarium oxysporum f.sp. ricini &#8211; Springer<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Fusarium oxysporum f. sp. ricini (F. o. ricini) is a ubiquitous soil borne pathogen which causes wilt disease on castor (Ricinus communis L). Rapid and reliable detection of the pathogen is essential for undertaking appropriate and timely disease management measures. Identification based on cultural, morphological characteristics and pathogenicity tests are time-consuming and laborious. <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1252\">Development of specific markers for identification of Indian isolates of Fusarium oxysporum f.sp. ricini<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[14,30,15,3,11,10],"tags":[23,16,122,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1252"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1252"}],"version-history":[{"count":9,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1252\/revisions"}],"predecessor-version":[{"id":1262,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1252\/revisions\/1262"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1252"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1252"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}