{"id":1302,"date":"2013-01-16T11:55:44","date_gmt":"2013-01-16T10:55:44","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=1302"},"modified":"2013-01-16T12:35:49","modified_gmt":"2013-01-16T11:35:49","slug":"development-of-the-molecular-methods-for-rapid-detection-and-differentiation-of-fusarium-oxysporum-and-f-oxysporum-f-sp-niveum-in-taiwan","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=1302","title":{"rendered":"Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan"},"content":{"rendered":"<div class=\"textimage\">\n<p class=\"textimage\"><a href=\"http:\/\/sppadbase.ipp.cnr.it\/?attachment_id=1303\" rel=\"attachment wp-att-1303\"><img decoding=\"async\" loading=\"lazy\" src=\"http:\/\/sppadbase.ipp.cnr.it\/wordpress\/wp-content\/uploads\/2013\/01\/1-s2.0-S1871678410004383-gr5-239x300.jpg\" alt=\"Microsoft Word - 2.doc\" width=\"239\" height=\"300\" class=\"alignnone size-medium wp-image-1303\" srcset=\"https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/1-s2.0-S1871678410004383-gr5-239x300.jpg 239w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/1-s2.0-S1871678410004383-gr5-119x150.jpg 119w, https:\/\/sppadbase.ipsp.cnr.it\/wp-content\/uploads\/2013\/01\/1-s2.0-S1871678410004383-gr5.jpg 394w\" sizes=\"(max-width: 239px) 100vw, 239px\" \/><\/a><em>Fusarium<\/em> wilt, caused by <em>Fusarium oxysporum<\/em> Fo, is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, <em>F. oxysporum<\/em> f. sp. <em>niveum<\/em> Fon is the damaging pathogen causing <em>Fusarium<\/em> wilt disease on watermelon, whereas F. oxysporum f. sp. cubense is the pathogen that infects banana. A rapid and reliable pathogen identification or disease diagnosis is essential for the integrated disease management practices in many crops. In this study, two new primer sets, Fon-1\/Fon-2 and FnSc-1\/FnSc-2, were developed to differentiate Fon and Fo, respectively. The PCR method using the novel primer sets has high sensitivity to detect Fon when the DNA concentration was as low as 0.01\u00a0pg or when the conidia number was as few as 5. In comparison with the published primer set, the Fon-1\/Fon-2 primer set, derived from the sequence of OP-M12 random primer-amplified fragment, produced a 174\u00a0bp DNA fragment, and was more specific to Fon in Taiwan. In addition, with optimized PCR parameters, the molecular method using the Fon-1\/Fon-2 primer set could directly detect Fon even when watermelon samples were collected in its early stage of disease development.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Ying-Hong Lin, Kan-Shu Chen, Jing-Yi Chang, Yu-Ling Wan, Ching-Chi Hsu, Jenn-Wen Huang, Pi-Fang Linda Chang<\/strong><br \/>\nNew Biotechnology<br \/>\nVolume 27, Issue 4, 30 September 2010, Pages 409\u2013418<\/p>\n<p>http:\/\/dx.doi.org\/10.1016\/j.nbt.2010.05.005<\/p>\n<blockquote><p>Please note that the <em><strong>New Biotecnology<\/strong><\/em> is not included in the monitored journal list, so the primer set specific to <em>F. oxysporum<\/em> f. sp. <em>niveum<\/em> will not be loaded into the database.<br \/>\nAt your convenience, I report here the Fon primer sequences:<\/p>\n<p>Fon-1\/Fon-2a\tCGATTAGCGAAGACATTCACAAGACT\/ACGGTCAAGAAGATGCAGGGTAAAGGT<\/p>\n<p>If more papers on primers specific to phytopathogens will be published on this journal, I will consider its inclusion in the monitored journal list.\n<\/p><\/blockquote>\n<p>via <a href='http:\/\/www.sciencedirect.com\/science\/article\/pii\/S1871678410004383'>ScienceDirect.com &#8211; New Biotechnology &#8211; Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"\n<p class=\"textimage\">Fusarium wilt, caused by Fusarium oxysporum Fo, is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum Fon is the damaging pathogen causing Fusarium wilt disease on watermelon, whereas F. oxysporum f. sp. cubense <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=1302\">Development of the molecular methods for rapid detection and differentiation of Fusarium oxysporum and F. oxysporum f. sp. niveum in Taiwan<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[14,30,15,3,11,10],"tags":[23,98,16,122,116,58,19,59],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1302"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1302"}],"version-history":[{"count":16,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1302\/revisions"}],"predecessor-version":[{"id":1319,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/1302\/revisions\/1319"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1302"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1302"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1302"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}