{"id":728,"date":"2012-04-10T11:09:45","date_gmt":"2012-04-10T10:09:45","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=728"},"modified":"2012-04-11T13:46:16","modified_gmt":"2012-04-11T12:46:16","slug":"scar-based-real-time-taqman-pcr-for-early-detection-of-fusarium-oxysporum-in-melon-seedlings-under-greenhouse-nursery-conditions","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=728","title":{"rendered":"SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions"},"content":{"rendered":"

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Isolates of the fungus Fusarium oxysporum cause some of the most devastating plant diseases, leading to significant crop losses in both the greenhouse nursery and field environments. Because of this, tools that permit early pathogen detection are essential. This study develops such a tool. A specific primer set (FOX S and FOX R) and TaqMan probe (FOX TM) for F. oxysporum quantification were developed from an SCAR marker derived from RAPD-PCR. This system was used to monitor the presence of F. oxysporum in the substrate and the vegetative tissue of melon seedlings growing under greenhouse conditions. Detection and quantification data were obtained within 48 h with the new technique, compared to 5\u20136 days for the classical plate culture method, while also providing greater specificity and sensitivity.<\/p>\n

Rub\u00e9n L\u00f3pez-Mond\u00e9jar, Robert Beaulieu, Margarita Ros, Jos\u00e9 Antonio Pascual<\/strong>
\nCrop Protection
\nVolume 33, March 2012, Pages 1\u20136<\/p>\n

ScienceDirect.com – Crop Protection – SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions<\/a>.<\/p>\n

DATABASE RECORD: 524<\/p>\n","protected":false},"excerpt":{"rendered":"<\/p>\n

Isolates of the fungus Fusarium oxysporum cause some of the most devastating plant diseases, leading to significant crop losses in both the greenhouse nursery and field environments. Because of this, tools that permit early pathogen detection are essential. This study develops such a tool. A specific primer set (FOX S and FOX R) and . . . → Read More: SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[14,30,15,3,10],"tags":[23,16,116,19],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/728"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=728"}],"version-history":[{"count":16,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/728\/revisions"}],"predecessor-version":[{"id":757,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/728\/revisions\/757"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=728"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=728"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=728"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}