{"id":801,"date":"2012-04-20T11:27:02","date_gmt":"2012-04-20T10:27:02","guid":{"rendered":"http:\/\/sppadbase.ipp.cnr.it\/?p=801"},"modified":"2012-04-20T15:42:21","modified_gmt":"2012-04-20T14:42:21","slug":"development-of-real-time-pcr-assay-using-taqman-probe-for-detection-and-quantification-of-rosellinia-necatrix-in-plant-and-soil","status":"publish","type":"post","link":"https:\/\/sppadbase.ipsp.cnr.it\/?p=801","title":{"rendered":"Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil"},"content":{"rendered":"<p>See on <a style='font-weight: bold; font-size: 18px;' href='http:\/\/www.scoop.it\/t\/pcr-primers-for-phytopathogenic-fungi\/p\/1581129784\/development-of-real-time-pcr-assay-using-taqman-probe-for-detection-and-quantification-of-rosellinia-necatrix-in-plant-and-soil'>Scoop.it<\/a> &#8211; <a href='http:\/\/www.scoop.it\/t\/pcr-primers-for-phytopathogenic-fungi'>PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI<\/a><\/p>\n<div class=\"textimage\">\n<p class=\"textimage\"><img src='http:\/\/img.scoop.it\/728ZSx1zySA5y1kliXPo3jl72eJkfbmt4t8yenImKBXEejxNn4ZJNZ2ss5Ku7Cxt'\/>We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low as 1 fg of template DNA. Using the real-time PCR assays with the TaqMan probes, we were able to quantify R. necatrix DNA in naturally diseased roots of Japanese pear and in artificially infested soil samples. Although the new assays were inadequate for use with naturally infested soil samples, nested PCR procedures improved the detectability of the new assays.<\/p>\n<div class=\"clearer\"><\/div>\n<p><strong>Masahiro Shishido, Itsuki Kubota and Hitoshi Nakamura<\/strong><br \/>\nJOURNAL OF GENERAL PLANT PATHOLOGY<br \/>\nVolume 78, Number 2, 115-120<br \/>\n<a href=\"http:\/\/dx.doi.org\/10.1007\/s10327-012-0366-x\">http:\/\/dx.doi.org\/10.1007\/s10327-012-0366-x<\/a><\/p>\n<p>See on <a href='http:\/\/www.springerlink.com\/content\/a4v5n4100x2j5101\/'>www.springerlink.com<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>See on Scoop.it &#8211; PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI<\/p>\n<p class=\"textimage\">We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low <span style=\"color:#777\"> . . . &rarr; Read More: <a href=\"https:\/\/sppadbase.ipsp.cnr.it\/?p=801\">Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil<\/a><\/span><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[15,3,11,10,36,37],"tags":[23,16,116,19,127],"_links":{"self":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/801"}],"collection":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=801"}],"version-history":[{"count":6,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/801\/revisions"}],"predecessor-version":[{"id":829,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=\/wp\/v2\/posts\/801\/revisions\/829"}],"wp:attachment":[{"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=801"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=801"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sppadbase.ipsp.cnr.it\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=801"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}