Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum

Acremonium cucurbitacearum is a soil-borne pathogen that causes collapse of muskmelon and watermelon plants. Cluster analysis based on RAPD patterns, obtained from use of 25 primers, divided isolates of A. cucurbitacearum from Spain and USA into two major groups. Most isolates from the USA fell into group 1, however, genetic similarity was not . . . → Read More: Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum

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A Real-Time PCR Assay for Detection and Quantification of Verticillium dahliae in Spinach Seed

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verticillium dahliae is a soilborne fungus that causes Verticillium wilt on multiple crops in central coastal California. Although spinach crops grown in this region for fresh and processing commercial production do not display Verticillium wilt symptoms, spinach seeds produced in the United . . . → Read More: A Real-Time PCR Assay for Detection and Quantification of Verticillium dahliae in Spinach Seed

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Development of an Assay for Rapid Detection and Quantification of Verticillium dahliae in Soil

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verticillium dahliae is responsible for Verticillium wilt on a wide range of hosts, including strawberry, on which low soil inoculum densities can cause significant crop loss. Determination of inoculum density is currently done by soil plating but this can take 6 to . . . → Read More: Development of an Assay for Rapid Detection and Quantification of Verticillium dahliae in Soil

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Molecular Identification of Two Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cepae

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Fusarium oxysporum f. sp. cepae, which causes basal rot of onion, consists of seven vegetative compatibility groups (VCGs 0420 to 0426) and several single-member VCGs (SMVs). F. oxysporum f. sp. cepae populations in South Africa and Colorado each consist of one main . . . → Read More: Molecular Identification of Two Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cepae

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PCR-Based Detection of Cephalosporium gramineum in Winter Wheat

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A polymerase chain reaction (PCR) assay was developed amplifying a 496-bp fragment of the internal transcribed spacer region of Cephalosporium gramineum genomic DNA at concentrations of 100 fg/μl. Winter wheat seed and seedlings were collected from field plots where C. gramineum was . . . → Read More: PCR-Based Detection of Cephalosporium gramineum in Winter Wheat

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