Phytophthora sojae is a devastating pathogen that causes soybean Phytophthora root rot. This study reports the development of a loop-mediated isothermal amplification (LAMP) assay targeting the A3aPro element for visual detection of P. sojae. The A3aPro-LAMP assay efficiently amplified the target element in < 80 min at 64 °C and was evaluated for . . . → Read More: Development of a loop-mediated isothermal amplification assay for detection of Phytophthora sojae
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The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, we describe a padlock probe (PLP)-based multiplex method of detection and . . . → Read More: A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes  Comments are closed  journal article, New Primer Set, paper, Phytophthora  diagnostics, pathogens, PCR, Phytophthora, plant pathology, primers See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, . . . → Read More: A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes Comments are closed journal article, paper, Phytophthora diagnostics, fungi, pathogens, Phytophthora, plant pathology Via Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI In the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a . . . → Read More: Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum |
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