New Primer Set « The Database of PCR Primers for Phytopathogenic Agents

Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

By Stefano Ghignone, on April 17th, 2012

Via Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Fusarium wilt, caused by Fusarium oxysporum f. sp. dianthi (Fod), is the most important carnation disease worldwide. The knowledge of the diversity of the soil population of the pathogen is essential for the choice of suitable resistant cultivars. We examined the genetic diversity . . . → Read More: Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

By Stefano Ghignone, on April 17th, 2012

Via Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

In the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a . . . → Read More: Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides

By Stefano Ghignone, on April 10th, 2012

Via Scoop.it – PCR PRIMERS FOR PHYTOPATHOGENIC FUNGI

Identification of the causal agent for anthracnose caused by C. acutatum and C. gloeosporioides based on morphological and cultural criteria is problematic as both are morphologically and genetically diverse. To evaluate a qualitative molecular method to readily distinguish between these two species, Restriction fragment length . . . → Read More: A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides

Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene.

By Stefano Ghignone, on April 10th, 2012

Detection of Fusarium solani causal agent of wilt and rots in many plant species are difficult if based only on morphological characteristics. Beside this, morphological discrimination requires special skill and the expertise of taxonomists or specialists. To simplify the detection and discrimination of F. solani from other Fusarium species, end-point polymerase chain . . . → Read More: Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene.

SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

By Stefano Ghignone, on April 10th, 2012

Isolates of the fungus Fusarium oxysporum cause some of the most devastating plant diseases, leading to significant crop losses in both the greenhouse nursery and field environments. Because of this, tools that permit early pathogen detection are essential. This study develops such a tool. A specific primer set (FOX S and FOX R) and . . . → Read More: SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

The Database of PCR Primers for Phytopathogenic Agents

Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides

Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene.

SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

Live Counts