Ceratocystis platani is a quarantine fungal pathogen agent of canker stain, a destructive disease affecting Platanus. Despite its diagnosis being critical for disease control, there is still no effective diagnostic tool as all known mycological and biological detection assays are problematical. In this study we developed highly effective Real-Time PCR methods based on . . . → Read More: Development of Real-Time PCR for in wood-detection of Ceratocystis platani, the agent of canker stain of Platanus spp.
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 Comments are closed  Ceratocystis, Ceratocystis platani, journal article, New Primer Set, paper  Ceratocystis platani, diagnostics, fungi, pathogens, PCR, plant pathology, primers Rhizomania is the most destructive disease in sugar beet throughout the world. The disease is caused by Beet necrotic yellow vein virus (BNYVV). Polymyxa betae (Keskin) is the only known vector of BNYVV, for transmission of the virus between the plants. Developing molecular diagnostic methods has a major impact on forecasting and epidemiological . . . → Read More: Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae Comments are closed journal article, New Primer Set, paper, Polymyxa, Polymyxa betae diagnostics, fungi, pathogens, PCR, plant pathology, Polymyxa betae, primers
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques. Methods We analyzed 2,085 18S rRNA gene sequences from the SILVA database for assay design. We generated and quantified plasmid . . . → Read More: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay Botrytis cinerea affects grape quality and yield, and can be difficult to manage due in part to non-symptomatic, quiescent infection in berry development. The aim of this study was to develop a dual system for the detection, isolation and quantification of B. cinerea. After three days of samples replication on the modified selective . . . → Read More: Early detection of gray mold in grape using conventional and molecular methods Comments are closed Botrytis, Botrytis cinerea, journal article, New Primer Set, paper, pathogens Botrytis cinerea, diagnostics, fungi, pathogens, PCR, primers [ Eiken GENOME SITE ] – The principle of LAMP method. “LAMP” which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on . . . → Read More: The principle of LAMP method Comments are closed Loop-mediated Isothermal Amplification (LAMP), Technology diagnostics, LAMP, methods, pathogens, PCR, plant pathology, primers |
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