Ceratocystis platani is a quarantine fungal pathogen agent of canker stain, a destructive disease affecting Platanus. Despite its diagnosis being critical for disease control, there is still no effective diagnostic tool as all known mycological and biological detection assays are problematical. In this study we developed highly effective Real-Time PCR methods based on . . . → Read More: Development of Real-Time PCR for in wood-detection of Ceratocystis platani, the agent of canker stain of Platanus spp.
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 Comments are closed  Ceratocystis, Ceratocystis platani, journal article, New Primer Set, paper  Ceratocystis platani, diagnostics, fungi, pathogens, PCR, plant pathology, primers Rhizomania is the most destructive disease in sugar beet throughout the world. The disease is caused by Beet necrotic yellow vein virus (BNYVV). Polymyxa betae (Keskin) is the only known vector of BNYVV, for transmission of the virus between the plants. Developing molecular diagnostic methods has a major impact on forecasting and epidemiological . . . → Read More: Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae Comments are closed journal article, New Primer Set, paper, Polymyxa, Polymyxa betae diagnostics, fungi, pathogens, PCR, plant pathology, Polymyxa betae, primers [ Eiken GENOME SITE ] – The principle of LAMP method. “LAMP” which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd. It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on . . . → Read More: The principle of LAMP method Comments are closed Loop-mediated Isothermal Amplification (LAMP), Technology diagnostics, LAMP, methods, pathogens, PCR, plant pathology, primers The combined data set of the acl1 and tef-1α gene sequences of 61 fungal strains assigned to Fusarium tricinctum, Fusarium avenaceum, Fusarium acuminatum, Fusarium arthrosporioides, Fusarium flocciferum and Fusarium torulosum were used to study the phylogenetic relations between taxa. F. tricinctum, F. acuminatum and F. avenaceum formed distinct clades. Members of the F. . . . → Read More: ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, we describe a padlock probe (PLP)-based multiplex method of detection and . . . → Read More: A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes Comments are closed journal article, New Primer Set, paper, Phytophthora diagnostics, pathogens, PCR, Phytophthora, plant pathology, primers |
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