Plasmopara halstedii, the causal agent of downy mildew of sunflower, is an oomycete listed as a quarantine pathogen. This obligate parasite resides in a quiescent state in seeds of sunflower and can be spread from seed production areas to areas of crop production by international seed trade. To prevent the spread or the . . . → Read More: An Optimized Duplex Real-Time PCR Tool for Sensitive Detection of the Quarantine Oomycete Plasmopara halstedii in Sunflower Seeds
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Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for . . . → Read More: Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum A protocol using real-time polymerase chain reaction (PCR) for the direct detection and quantification of Plasmodiophora brassicae in soil samples was developed and used on naturally and artificially infested soil samples containing different concentrations of P. brassicae. Species-specific primers and a TaqMan fluorogenic probe were designed to amplify a small region of P. . . . → Read More: In-field distribution of Plasmodiophora brassicae measured using quantitative real-time PCR See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI The overall aim of this study was to develop a new, reliable and rapid diagnostic assay for differentiating six European Armillaria species based on variation in their elongation factor-1 alpha (EF-1 α) gene sequences and to verify a set of species-specific primers . . . → Read More: Development and verification of a diagnostic assay based on EF-1 α for the identification of Armillaria species in Northern Europe  Comments are closed  Armillaria, journal article, Next Generation Sequencing, paper, pathogens  Armillaria, diagnostics, fungi, pathogens, PCR, plant pathology, primers Full-length coding sequences of the β-tubulin gene (tubA) were PCR-amplified and sequenced from 42 Phaeosphaeria isolates, including 16 P. nodorum and 23 P. avenaria species from cereals, two Polish isolates from rye (Secale cereale L.), and one isolate from dallis grass (Paspalum dilatatum Poir). A tubA gene of size 1556 bp was identified . . . → Read More: Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria |
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