Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria

Full-length coding sequences of the β-tubulin gene (tubA) were PCR-amplified and sequenced from 42 Phaeosphaeria isolates, including 16 P. nodorum and 23 P. avenaria species from cereals, two Polish isolates from rye (Secale cereale L.), and one isolate from dallis grass (Paspalum dilatatum Poir). A tubA gene of size 1556 bp was identified . . . → Read More: Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria

Share

Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines

Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have been hampered by its common occurrence in the internal tissue of apparently healthy vines. Development of a molecular marker for a single strain would overcome . . . → Read More: Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines

Share

In Vivo Monitoring of Obligate Biotrophic Pathogen Growth by Kinetic PCR

The plant kingdom is constantly challenged by a battery of evolving pathogens. New species or races of pathogens are discovered on crops that were initially bred for disease resistance, and globalization is facilitating the movement of exotic pests. Among these pests, obligate biotrophic parasites make up some of the most damaging groups and . . . → Read More: In Vivo Monitoring of Obligate Biotrophic Pathogen Growth by Kinetic PCR

Share

Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum

Acremonium cucurbitacearum is a soil-borne pathogen that causes collapse of muskmelon and watermelon plants. Cluster analysis based on RAPD patterns, obtained from use of 25 primers, divided isolates of A. cucurbitacearum from Spain and USA into two major groups. Most isolates from the USA fell into group 1, however, genetic similarity was not . . . → Read More: Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum

Share

A Real-Time PCR Assay for Detection and Quantification of Verticillium dahliae in Spinach Seed

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verticillium dahliae is a soilborne fungus that causes Verticillium wilt on multiple crops in central coastal California. Although spinach crops grown in this region for fresh and processing commercial production do not display Verticillium wilt symptoms, spinach seeds produced in the United . . . → Read More: A Real-Time PCR Assay for Detection and Quantification of Verticillium dahliae in Spinach Seed

Share