Fusarium species can produce mycotoxins, which can contaminate cereal-based food producing adverse effects for human and animal health. In recent years, the importance of Fusarium poae has increased within the Fusarium head blight complex. Fusarium poae is known to produce trichothecenes, especially nivalenol, a potent mycotoxin able to cause a variety of toxic . . . → Read More: Development of a PCR assay to detect the potential production of nivalenol in Fusarium poae
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Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have been hampered by its common occurrence in the internal tissue of apparently healthy vines. Development of a molecular marker for a single strain would overcome . . . → Read More: Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI Unlike bacterial toxins that are primarily peptides and are therefore encoded by a single gene, fungal toxins such as the aflatoxins are multi-ring structures and therefore require a sequence of structural genes for their biological synthesis. There is therefore no specific PCR . . . → Read More: PCR detection of aflatoxin producing fungi and its limitations  Comments are closed  aflatoxin, Aspergillus, mycotoxins, paper, pathogens, review  aflatoxins, contamination, diagnostics, fungi, mycotoxins, pathogens, plant pathology See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a real-time PCR (qPCR) assay . . . → Read More: Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI A multiplex real-time PCR (qPCR) method to quantify aflatoxin, ochratoxin A (OTA) and patulin producing molds in foods was developed. For this, the primer pairs F/R-omt, F/R-npstr and F/R-idhtrb and the TaqMan probes, OMTprobe, NPSprobe and IDHprobe targeting the omt-1, otanpsPN and . . . → Read More: Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods |
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