pathogens « The Database of PCR Primers for Phytopathogenic Agents

Development of an Assay for Rapid Detection and Quantification of Verticillium dahliae in Soil

By Stefano Ghignone, on May 14th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verticillium dahliae is responsible for Verticillium wilt on a wide range of hosts, including strawberry, on which low soil inoculum densities can cause significant crop loss. Determination of inoculum density is currently done by soil plating but this can take 6 to . . . → Read More: Development of an Assay for Rapid Detection and Quantification of Verticillium dahliae in Soil

Molecular Identification of Two Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cepae

By Stefano Ghignone, on May 14th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Fusarium oxysporum f. sp. cepae, which causes basal rot of onion, consists of seven vegetative compatibility groups (VCGs 0420 to 0426) and several single-member VCGs (SMVs). F. oxysporum f. sp. cepae populations in South Africa and Colorado each consist of one main . . . → Read More: Molecular Identification of Two Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cepae

PCR-Based Detection of Cephalosporium gramineum in Winter Wheat

By Stefano Ghignone, on May 14th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

A polymerase chain reaction (PCR) assay was developed amplifying a 496-bp fragment of the internal transcribed spacer region of Cephalosporium gramineum genomic DNA at concentrations of 100 fg/μl. Winter wheat seed and seedlings were collected from field plots where C. gramineum was . . . → Read More: PCR-Based Detection of Cephalosporium gramineum in Winter Wheat

Detection of Phomopsis sclerotioides in Commercial Cucurbit Field Soil by Nested Time-Release PCR

By Stefano Ghignone, on May 14th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

A polymerase chain reaction (PCR)-based molecular method to detect Phomopsis sclerotioides in soil was developed using a species-specific primer pair. To improve sensitivity of the detection, three PCR techniques were used; namely, nested PCR using the primer pair internal transcribed spacer (ITS)1 . . . → Read More: Detection of Phomopsis sclerotioides in Commercial Cucurbit Field Soil by Nested Time-Release PCR

Identification of the causal agent of Verticillium wilt of winter oilseed rape in Sweden, V. longisporum

By Stefano Ghignone, on May 14th, 2012

ScienceDirect.com – Mycological Research – Identification of the causal agent of Verticillium wilt of winter oilseed rape in Sweden, V. longisporum.

Morphological characters and genetic relationships were studied among 36 Verticillium isolates derived from different host species. Conidial length varied from 3.5 μm to 12 μm and the DNA content ranged from 0.028 pg . . . → Read More: Identification of the causal agent of Verticillium wilt of winter oilseed rape in Sweden, V. longisporum

The Database of PCR Primers for Phytopathogenic Agents

Development of an Assay for Rapid Detection and Quantification of Verticillium dahliae in Soil

Molecular Identification of Two Vegetative Compatibility Groups of Fusarium oxysporum f. sp. cepae

PCR-Based Detection of Cephalosporium gramineum in Winter Wheat

Detection of Phomopsis sclerotioides in Commercial Cucurbit Field Soil by Nested Time-Release PCR

Identification of the causal agent of Verticillium wilt of winter oilseed rape in Sweden, V. longisporum

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