Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene.

Detection of Fusarium solani causal agent of wilt and rots in many plant species are difficult if based only on morphological characteristics. Beside this, morphological discrimination requires special skill and the expertise of taxonomists or specialists. To simplify the detection and discrimination of F. solani from other Fusarium species, end-point polymerase chain . . . → Read More: Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene.

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SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

Isolates of the fungus Fusarium oxysporum cause some of the most devastating plant diseases, leading to significant crop losses in both the greenhouse nursery and field environments. Because of this, tools that permit early pathogen detection are essential. This study develops such a tool. A specific primer set (FOX S and FOX R) and . . . → Read More: SCAR-based real-time TaqMan PCR for early detection of Fusarium oxysporum in melon seedlings under greenhouse nursery conditions

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Application of High-Throughput DNA Sequencing in Phytopathology

Application of High-Throughput DNA Sequencing in Phytopathology Annual Review of Phytopathology Vol. 49: 87-105 (Volume publication date September 2011) DOI: 10.1146/annurev-phyto-072910-095408 David J. Studholme, Rachel H. Glover, and Neil Boonham

A must-read review on Next-Generation Sequencing methods applied in Plant Pathology. Both Genomic and Transcriptomic aspects are treated, and the associated computational and bioinformatics . . . → Read More: Application of High-Throughput DNA Sequencing in Phytopathology

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