paper « The Database of PCR Primers for Phytopathogenic Agents

Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low . . . → Read More: Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Fusarium oxysporum f. sp. melonis (FOM) is the cause of vascular wilting of melon, the most severe infectious disease of this cucurbit. Four races (0, 1, 2, and 1,2) of FOM have been identified all of which occur in Italy. A PCR assay . . . → Read More: Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Aflatoxins are secondary metabolites produced mainly by Aspergillus species growing in foodstuffs. Because aflatoxins have important health effects, the detection of early contamination of foods by aflatoxigenic molds should be useful. In the present work, a reliable conventional PCR method for detecting . . . → Read More: Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

By Stefano Ghignone, on April 17th, 2012

Via Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Fusarium wilt, caused by Fusarium oxysporum f. sp. dianthi (Fod), is the most important carnation disease worldwide. The knowledge of the diversity of the soil population of the pathogen is essential for the choice of suitable resistant cultivars. We examined the genetic diversity . . . → Read More: Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

By Stefano Ghignone, on April 17th, 2012

Via Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

In the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a . . . → Read More: Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

The Database of PCR Primers for Phytopathogenic Agents

Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

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