PCR detection of aflatoxin producing fungi and its limitations

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Unlike bacterial toxins that are primarily peptides and are therefore encoded by a single gene, fungal toxins such as the aflatoxins are multi-ring structures and therefore require a sequence of structural genes for their biological synthesis. There is therefore no specific PCR . . . → Read More: PCR detection of aflatoxin producing fungi and its limitations

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Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

A multiplex real-time PCR (qPCR) method to quantify aflatoxin, ochratoxin A (OTA) and patulin producing molds in foods was developed. For this, the primer pairs F/R-omt, F/R-npstr and F/R-idhtrb and the TaqMan probes, OMTprobe, NPSprobe and IDHprobe targeting the omt-1, otanpsPN and . . . → Read More: Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

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Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Aflatoxins are secondary metabolites produced mainly by Aspergillus species growing in foodstuffs. Because aflatoxins have important health effects, the detection of early contamination of foods by aflatoxigenic molds should be useful. In the present work, a reliable conventional PCR method for detecting . . . → Read More: Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

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