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See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI The overall aim of this study was to develop a new, reliable and rapid diagnostic assay for differentiating six European Armillaria species based on variation in their elongation factor-1 alpha (EF-1 α) gene sequences and to verify a set of species-specific primers . . . → Read More: Development and verification of a diagnostic assay based on EF-1 α for the identification of Armillaria species in Northern Europe  Comments are closed  Armillaria, journal article, Next Generation Sequencing, paper, pathogens  Armillaria, diagnostics, fungi, pathogens, PCR, plant pathology, primers Full-length coding sequences of the β-tubulin gene (tubA) were PCR-amplified and sequenced from 42 Phaeosphaeria isolates, including 16 P. nodorum and 23 P. avenaria species from cereals, two Polish isolates from rye (Secale cereale L.), and one isolate from dallis grass (Paspalum dilatatum Poir). A tubA gene of size 1556 bp was identified . . . → Read More: Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have been hampered by its common occurrence in the internal tissue of apparently healthy vines. Development of a molecular marker for a single strain would overcome . . . → Read More: Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines The plant kingdom is constantly challenged by a battery of evolving pathogens. New species or races of pathogens are discovered on crops that were initially bred for disease resistance, and globalization is facilitating the movement of exotic pests. Among these pests, obligate biotrophic parasites make up some of the most damaging groups and . . . → Read More: In Vivo Monitoring of Obligate Biotrophic Pathogen Growth by Kinetic PCR Comments are closed journal article, Melampsora, New Primer Set, paper, pathogens diagnostics, fungi, Melampsora larici-populina, Melampsora medusae, pathogens, PCR, plant pathology, primers Acremonium cucurbitacearum is a soil-borne pathogen that causes collapse of muskmelon and watermelon plants. Cluster analysis based on RAPD patterns, obtained from use of 25 primers, divided isolates of A. cucurbitacearum from Spain and USA into two major groups. Most isolates from the USA fell into group 1, however, genetic similarity was not . . . → Read More: Molecular characterization and PCR detection of the melon pathogen Acremonium cucurbitacearum |
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