Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

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Fusarium oxysporum f. sp. melonis (FOM) is the cause of vascular wilting of melon, the most severe infectious disease of this cucurbit. Four races (0, 1, 2, and 1,2) of FOM have been identified all of which occur in Italy. A PCR assay . . . → Read More: Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

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Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

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Aflatoxins are secondary metabolites produced mainly by Aspergillus species growing in foodstuffs. Because aflatoxins have important health effects, the detection of early contamination of foods by aflatoxigenic molds should be useful. In the present work, a reliable conventional PCR method for detecting . . . → Read More: Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

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Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

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Fusarium wilt, caused by Fusarium oxysporum f. sp. dianthi (Fod), is the most important carnation disease worldwide. The knowledge of the diversity of the soil population of the pathogen is essential for the choice of suitable resistant cultivars. We examined the genetic diversity . . . → Read More: Molecular analysis of Spanish populations of Fusarium oxysporum f. sp. dianthi demonstrates a high genetic diversity and identifies virulence groups in races 1 and 2 of the pathogen

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Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

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In the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a . . . → Read More: Detection of mRNA by reverse-transcription PCR as an indicator of viability in Phytophthora ramorum

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A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides

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Identification of the causal agent for anthracnose caused by C. acutatum and C. gloeosporioides based on morphological and cultural criteria is problematic as both are morphologically and genetically diverse. To evaluate a qualitative molecular method to readily distinguish between these two species, Restriction fragment length . . . → Read More: A rapid qualitative molecular method for the identification of Colletotrichum acutatum and C. gloeosporioides

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