New Primer Set « The Database of PCR Primers for Phytopathogenic Agents

Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

A multiplex real-time PCR (qPCR) method to quantify aflatoxin, ochratoxin A (OTA) and patulin producing molds in foods was developed. For this, the primer pairs F/R-omt, F/R-npstr and F/R-idhtrb and the TaqMan probes, OMTprobe, NPSprobe and IDHprobe targeting the omt-1, otanpsPN and . . . → Read More: Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verrucosidin, which is a tremorgenic mycotoxin responsible for neurological diseases, has been detected in different dry-ripened foods as consequence of the growth of toxigenic molds. To improve food safety, the presence of verrucosidin producing molds in these kind foods should be quantified. . . . → Read More: Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low . . . → Read More: Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Fusarium oxysporum f. sp. melonis (FOM) is the cause of vascular wilting of melon, the most severe infectious disease of this cucurbit. Four races (0, 1, 2, and 1,2) of FOM have been identified all of which occur in Italy. A PCR assay . . . → Read More: Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Aflatoxins are secondary metabolites produced mainly by Aspergillus species growing in foodstuffs. Because aflatoxins have important health effects, the detection of early contamination of foods by aflatoxigenic molds should be useful. In the present work, a reliable conventional PCR method for detecting . . . → Read More: Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

The Database of PCR Primers for Phytopathogenic Agents

Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

Development of SCAR markers and PCR assay for Fusarium oxysporum f.sp. melonis race 2-specific detection

Development of a PCR Protocol To Detect Aflatoxigenic Molds in Food Products

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