Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

A multiplex real-time PCR (qPCR) method to quantify aflatoxin, ochratoxin A (OTA) and patulin producing molds in foods was developed. For this, the primer pairs F/R-omt, F/R-npstr and F/R-idhtrb and the TaqMan probes, OMTprobe, NPSprobe and IDHprobe targeting the omt-1, otanpsPN and . . . → Read More: Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods

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Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verrucosidin, which is a tremorgenic mycotoxin responsible for neurological diseases, has been detected in different dry-ripened foods as consequence of the growth of toxigenic molds. To improve food safety, the presence of verrucosidin producing molds in these kind foods should be quantified. . . . → Read More: Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

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