Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum

Phaeomoniella chlamydospora and Phaeoacremonium aleophilum are the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for . . . → Read More: Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum

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Development and verification of a diagnostic assay based on EF-1 α for the identification of Armillaria species in Northern Europe

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

The overall aim of this study was to develop a new, reliable and rapid diagnostic assay for differentiating six European Armillaria species based on variation in their elongation factor-1 alpha (EF-1 α) gene sequences and to verify a set of species-specific primers . . . → Read More: Development and verification of a diagnostic assay based on EF-1 α for the identification of Armillaria species in Northern Europe

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Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria

Full-length coding sequences of the β-tubulin gene (tubA) were PCR-amplified and sequenced from 42 Phaeosphaeria isolates, including 16 P. nodorum and 23 P. avenaria species from cereals, two Polish isolates from rye (Secale cereale L.), and one isolate from dallis grass (Paspalum dilatatum Poir). A tubA gene of size 1556 bp was identified . . . → Read More: Sequence diversity of β-tubulin (tubA) gene in Phaeosphaeria nodorum and P. avenaria

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Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines

Petri disease causes decline of grapevines worldwide. The grapevine endophyte Phaeomoniella chlamydospora is the most important fungal pathogen associated with this disease. Epidemiological studies of this pathogen have been hampered by its common occurrence in the internal tissue of apparently healthy vines. Development of a molecular marker for a single strain would overcome . . . → Read More: Development of an isolate-specific marker for tracking Phaeomoniella chlamydospora infection in grapevines

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In Vivo Monitoring of Obligate Biotrophic Pathogen Growth by Kinetic PCR

The plant kingdom is constantly challenged by a battery of evolving pathogens. New species or races of pathogens are discovered on crops that were initially bred for disease resistance, and globalization is facilitating the movement of exotic pests. Among these pests, obligate biotrophic parasites make up some of the most damaging groups and . . . → Read More: In Vivo Monitoring of Obligate Biotrophic Pathogen Growth by Kinetic PCR

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