By Stefano Ghignone, on May 9th, 2012 See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, . . . → Read More: A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes
By Stefano Ghignone, on April 24th, 2012 See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
Unlike bacterial toxins that are primarily peptides and are therefore encoded by a single gene, fungal toxins such as the aflatoxins are multi-ring structures and therefore require a sequence of structural genes for their biological synthesis. There is therefore no specific PCR . . . → Read More: PCR detection of aflatoxin producing fungi and its limitations
By Stefano Ghignone, on April 20th, 2012 See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a real-time PCR (qPCR) assay . . . → Read More: Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize
By Stefano Ghignone, on April 20th, 2012 See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
A multiplex real-time PCR (qPCR) method to quantify aflatoxin, ochratoxin A (OTA) and patulin producing molds in foods was developed. For this, the primer pairs F/R-omt, F/R-npstr and F/R-idhtrb and the TaqMan probes, OMTprobe, NPSprobe and IDHprobe targeting the omt-1, otanpsPN and . . . → Read More: Development of a multiplex real-time PCR to quantify aflatoxin, ochratoxin A and patulin producing molds in foods
By Stefano Ghignone, on April 20th, 2012 See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI
Verrucosidin, which is a tremorgenic mycotoxin responsible for neurological diseases, has been detected in different dry-ripened foods as consequence of the growth of toxigenic molds. To improve food safety, the presence of verrucosidin producing molds in these kind foods should be quantified. . . . → Read More: Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods
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Live Counts Genera: 101
Species: 205
Primer sets: 575
References: 284
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