pathogens « The Database of PCR Primers for Phytopathogenic Agents

A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes

By Stefano Ghignone, on May 9th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

The genus Phytophthora consists of many species that cause important diseases in ornamental, agronomic, and forest ecosystems worldwide. Molecular methods have been developed for detection and identification of one or several species of Phytophthora in single or multiplex reactions. In this article, . . . → Read More: A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes

PCR detection of aflatoxin producing fungi and its limitations

By Stefano Ghignone, on April 24th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Unlike bacterial toxins that are primarily peptides and are therefore encoded by a single gene, fungal toxins such as the aflatoxins are multi-ring structures and therefore require a sequence of structural genes for their biological synthesis. There is therefore no specific PCR . . . → Read More: PCR detection of aflatoxin producing fungi and its limitations

Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Zearalenone (ZEA) is a mycotoxin produced by some species of Fusarium, especially by Fusarium graminearum and F. culmorum. ZEA induces hyperoestrogenic responses in mammals and can result in reproductive disorders in farm animals. In the present study, a real-time PCR (qPCR) assay . . . → Read More: Quantification of Fusarium graminearum and Fusarium culmorum by real-time PCR system and zearalenone assessment in maize

Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

Verrucosidin, which is a tremorgenic mycotoxin responsible for neurological diseases, has been detected in different dry-ripened foods as consequence of the growth of toxigenic molds. To improve food safety, the presence of verrucosidin producing molds in these kind foods should be quantified. . . . → Read More: Duplex real-time PCR method with internal amplification control for quantification of verrucosidin producing molds in dry-ripened foods

Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil

By Stefano Ghignone, on April 20th, 2012

See on Scoop.it – PCR PRIMERS FOR THE IDENTIFICATION OF PHYTOPATHOGENIC FUNGI

We developed real-time PCR assays using TaqMan probes to detect and quantify Rosellinia necatrix, the causal agent of white root rot in many plant species. Two sets of PCR primers and TaqMan probe indicated that their detection limits could be as low . . . → Read More: Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil